- #Serial cloner 2.6.1 program online serial#
- #Serial cloner 2.6.1 program online software#
- #Serial cloner 2.6.1 program online windows#
In contrast, MnP-2 showed high activity at pH 6 with optimum temperature at 65☌. Compared to wild type plants, transplastomic events expressing pectin lyase exhibited enzymatic activity above 58.5% of total soluble protein at neutral pH and 60☌. In general, mature plants showed more activity than young transplastomic plants. Enzymatic activity was evaluated to detect expression and functionality of both enzymes. Stable processing and expression of the RNA were confirmed by Northern blot analysis. Efficient gene integration into both inverted repeats of the intergenic region between rrn16S and 3'rps'12 was confirmed by Southern blot. Both genes are flanked by a synthetic rrn16S promoter and the 3'UTR from rbcL gene.
PES4 and pES5 vectors were derived from pPV111A plasmid and contain the PelA and MnP-2 synthetic genes, respectively. This report presents the stable expression of Pectin lyase ( PelA gene) and the first stable expression of manganese peroxidase ( MnP-2 gene) from the chloroplast genome. The high capacity of chloroplast genome response to integrate and express transgenes at high levels makes this technology a good option to produce proteins of interest. Victoria, Tamaulipas, MéxicoĬ Laboratorio de Biotecnología I, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito 1, Nuevo Campus Universitario, Chihuahua, México Ex hacienda El Canadá, General Escobedo, Nuevo León, Méxicoī Instituto de Ecología Aplicada, Universidad Autónoma de Tamaulipas, División del Golfo No. VAMP: Visualization and analysis of array-CGH, transcriptome and other molecular profilesīioinformatics 2006 22: 2066-2073 Posted on 9 9 Categories Miscellaneous Tags Analysis, CGH arrays, Molecular Profile, Transcriptome, VAMP, Visualisation Leave a comment on VAMP 2.2.Stable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplastĮdward Alexander Espinoza-Sánchez a, Marianela Hazel Álvarez-Hernández a, Jorge Ariel Torres-Castillo b, Quintín Rascón-Cruz c, Adriana Gutiérrez-Díez a, Francisco Zavala-García a, Sugey Ramona Sinagawa-García a, *Ī Laboratorio de Biotecnología, Campus de Ciencias Agropecuarias, Universidad Autónoma de Nuevo León, Francisco Villa S/N Col. VAMP offers many functionalities such as computing recurrent alterations, clustering profiles, crossing with clinical or biological annotations, performing syntenic projection onto the genome of another species.īioinformatics Laboratory of Institut Curie (Paris).
#Serial cloner 2.6.1 program online software#
The VAMP software (Visualization and Analysis of array-CGH, transcriptome and other Molecular Profiles)allows the visual comparison of the array-CGH profiles of a cohort of patients, or the confrontation of array-CGH, expression and loss of heterozygosity profiles.
#Serial cloner 2.6.1 program online serial#
:: MORE INFORMATION Posted on 0 0 Categories DNA / Genome Analysis Tags DNA Cloning, Sequence Analysis, SERIAL CLONER, Visualisation Leave a comment on SERIAL CLONER 2.6.1 – DNA Cloning, Sequence Analysis & Visualisation VAMP 2.2.8 – Visualisation and Analysis of CGH arrays, transcriptome and other Molecular Profiles Serial Cloner handles Annotations and Features both in the sequence and in the Graphic Map. Powerful graphical display tools and simple interfaces help the analysis and construction steps in a very intuitive way. Import from MacVector is also possible now. Serial Cloner also import files saved in the Vector NTI, ApE, pDRAW32 and GenBank formats. Serial Cloner reads and write DNA Strider-compatible files and import and export files in the universal FASTA format.
#Serial cloner 2.6.1 program online windows#
Serial Cloner has been developed to provide a light molecular biology software to both Macintosh and Windows users.
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